MNGS is helpful for identifying Cryptococcus species. was detected concurrently in 33.33 % (4/12). 36 Unusual morphological forms are rarely seen. The mNGS results of the CSF identified DNA sequences corresponding to Cryptococcus in 75 % of samples (9/12). India ink of the CSF is a traditional microscopic method of diagnosis, 35 although the sensitivity is poor in early infection, and may miss 1520 of patients with culture-positive cryptococcal meningitis. Antigen detection: Can be used on CSF or serum. Histopathology for detection of narrow-based budding yeasts in tissue can also be used. India ink staining and culture of the CSF were positive for Cryptococcus in 83.33 % (10/12) of the samples 100 % (11/11) were positive via CrAg EIA. Microscopy: India Ink can be performed on Cerebrospinal fluid (CSF ) to quickly visualize Cryptococcus cells under a microscope however, it has limited sensitivity. Whole cell (Wc) and cell body (Cb) measurements were taken for a minimum of 100 cells per. The patient had a CD4 count of 19 cells/µL, which prompted reflex screening for cryptococcal antigenaemia. gattii capsule, which is easily observed microscopically 90. Both CrAg LFA (in duplicate) and India ink staining of the CSF were negative, although on the CSF culture there was heavy growth of C. Cryptococcus Ag Screen, CSF Cryptococcus Antigen, Spinal Fluid India ink. We aimed to assess the diagnostic performance of mNGS of cerebrospinal fluid (CSF) for prediction of cryptococcal meningitis (CM).Ī comparative evaluation of mNGS (performed on CSF samples) and conventional methods, including India ink staining, culture for fungi and cryptococcal-antigen (CrAg) detection by enzyme immunoassay, was performed on 12 consecutive non-HIV-infected patients with chronic or subacute CM. India ink particles are repulsed by the C. Identify Cryptococcus as an etiological agent of meningitis. T2-35 Table 2: Clinical and laboratory profile of. In recent years, metagenomic next-generation sequencing (mNGS) has become widely used in medical microbiology to detect pathogen infection. Gram stain, India ink and culture findings of these patients have been depicted in Figures 13, respectively.
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